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Composite

Part:BBa_K1879002:Design

Designed by: Madeleine Stone   Group: iGEM16_Virginia   (2016-10-12)


mutant leucyl synthetase + terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 220
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1104
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 220
    Illegal AgeI site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutated leuS sequence was commercially synthesized and the terminator was in the iGEM kit.


Source

The original leuS gene originates in E. coli. The sequence for the mutated leuS gene was generated by our team using mutagenesis and docking software. The terminator in this part is from the iGEM kit (BBa_J61048).

References